Active Recombinant Full Length Human TRAC Protein, His Tagged

• Cat.No.: TRAC-1883H
• Product Overview: Human TRAC, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 1-Ser 115 (Accession # P01848-1). This protein carries a polyhistidine tag at the C-terminus. Predicted N-terminus: Ile 1

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-115 aa
• Description: The transmembrane protein, TCR, comprise of two disulphide-linked polypeptide chains: a α and β chain, a γ and δ chain. Each polypeptide chain consists of a variable and a constant region. TRAC is the constant region of T-cell receptor (TCR) alpha chain. TRAC is presented on the surface of T cell and recognized peptide-major histocompatibility (MH) (pMH) that are displayed by antigen presenting cells (APC). TRAC is participate in adaptive immune response and has been well-studied in T cell therapy.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human TRAC, His Tag at 5 μg/mL (100 μL/well) can bind TRAC Antibody with a linear range of 0.2-5 ng/mL.
• Molecular Mass: The protein has a calculated MW of 14.8 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.2 EU/μg by the LAL method/rFC method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: TRAC T cell receptor alpha constant [ Homo sapiens (human) ]
• Official Symbol: TRAC
• Synonyms: TRAC; T cell receptor alpha constant; IMD7; TCRA; TRCA
• Gene ID: 28755
• MIM: 186880
• UniProt ID: P01848