Recombinant Human Transcription Elongation Factor A (SII), 1, His-tagged

• Cat.No.: TCEA1-6769H
• Product Overview: Recombinant humanTCEA1 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: TCEA1 (Transcriptionelongation factor A protein 1) belongs to the TFS-II family. TCEA1 binds toPol II and functions to cleave the nascent transcript, thereby unlocking thecomplex and allowing transcription to continue. Localized to the nucleus,TCEA1 contains three independently-folding domains, all of which arenecessary for proper binding to Pol II. The arresting sites in DNA have theproperty of trapping a certain fraction of elongating RNA polymerases thatpass through, resulting in locked ternary complexes. Cleavage of the nascenttranscript by S-II allows the resumption of elongation from the new3"-terminus.
• Form: Liquid. In 20 mMTris-HCl buffer, pH7.5, 20% glycerol, 1mM DTT, 50mM NaCl
• Molecular Weight: 36.5kDa (325aa),confirmed by MALDI-TOF
• Purity: > 90% by SDS -PAGE
• Concentration: 1mg/ml (determinedby Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMEDEVV RFAKKMDKMV QKKNAAGALD LLKELKNIPM TLELLQSTRI GMSVNAIRKQSTDEEVTSLA KSLIKSWKKL LDGPSTEKDL DEKKKEPAIT SQNSPEAREE STSSGNVSNR KDETNARDTYVSSFPRAPST SDSVRLKCRE MLAAALRTGD DYIAIGADEE ELGSQIEEAI YQEIRNTDMK YKNRVRSRISNLKDAKNPNL RKNVLCGNIP PDLFARMTAE EMASDELKEM RKNLTKEAIR EHQMAKTGGT QTDLFTCGKCKKKNCTYTQV QTRSADEPMT TFVVCNECGN RWKFC
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: TCEA1transcription elongation factor A (SII), 1 [ Homo sapiens ]
• Official Symbol: TCEA1
• Synonyms: TCEA1; transcription elongationfactor A (SII), 1; GTF2S, TCEA; transcription elongation factor A protein 1;SII; TF2S; TFIIS
• Gene ID: 6917
• mRNA Refseq: NM_006756
• Protein Refseq: NP_006747
• MIM: 601425
• Uniprot ID: P23193
• Chromosome Location: 8q11.2
• Pathway: DNA Repair, organism-specificbiosystem; Dual incision reaction in TC-NER, organism-specific biosystem;Formation and Maturation of mRNA Transcript, organism-specific biosystem;Formation of HIV-1 elongation complex containing HIV-1 Tat, organism-specificbiosystem; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat,organism-specific biosystem
• Function: DNA binding; metal ion binding;translation elongation factor activity; zinc ion binding