Recombinant Human TRNA Phosphotransferase 1, His-tagged

• Cat.No.: TRPT1-1327H
• Product Overview: Recombinant human TRPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: TRPT1, also known as tRNA 2"-phosphotransferase 1, catalyzes the last step of tRNA splicing, the transfer of the splice junction 2"-phosphate from ligated tRNA to NAD to produce ADP-ribose 1"-2" cyclic phosphate.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 20% glycerol, 0.1 M NaCl.
• Molecular Weight: 30.3 kDa (277 aa), confirmed by MALDI-TOF
• Purity: > 95 % by SDS-PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMNFSGG GRQEAAGSRG RRAPRPREQD RDVQLSKALS YALRHGALKL GLPMGADGFV PLGTLLQLPQ FRGFSAEDVQ RVVDTNRKQR FALQLGDPST GLLIRANQGH SLQVPKLELM PLETPQALPP MLVHGTFWKH WPSILLKGLS CQGRTHIHLA PGLPGDPGII SGMRSHCEIA VFIDGPLALA DGIPFFRSAN GVILTPGNTD GFLLPKYFKE ALQLRPTRKP LSLAGDEETE CQSSPKHSSR ERRRIQQ
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: TRPT1 tRNA phosphotransferase 1 [ Homo sapiens ]
• Official Symbol: TRPT1
• Synonyms: TRPT1; tRNA phosphotransferase 1; MGC11134; tRNA 2"-phosphotransferase 1; OTTHUMP00000237038; OTTHUMP00000237040; OTTHUMP00000237336; OTTHUMP00000237493; tRNA splicing 2" phosphotransferase 1; NP_001028850.2; EC 2.7.1.160
• Gene ID: 83707
• mRNA Refseq: NM_001033678
• Protein Refseq: NP_001028850
• MIM: 610470
• UniProt ID: Q86TN4
• Chromosome Location: 11q13
• Function: tRNA 2"-phosphotransferase activity; transferase activity, transferring phosphorus-containing groups