Recombinant Human TRNT1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TRNT1-2182H
• Product Overview: TRNT1 MS Standard C13 and N15-labeled recombinant protein (NP_057084) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a CCA-adding enzyme which belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. This essential enzyme functions by catalyzing the addition of the conserved nucleotide triplet CCA to the 3' terminus of tRNA molecules. Mutations in this gene result in sideroblastic anemia with B-cell immunodeficiency, periodic fevers, and developmental delay. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 46.4 kDa
• AA Sequence: MKLQSPEFQSLFTEGLKSLTELFVK ENHELRIAGGAVRDLLNGVKPQDID FATTATPTQMKEMFQSAGIRMINNR GEKHGTITARLHEENFEITTLRIDV TTDGRHAEVEFTTDWQKDAERRDLT INSMFLGFDGTLFDYFNGYEDLKNK KVRFVGHAKQRIQEDYLRILRYFRF YGRIVDKPGDHDPETLEAIAENAKG LAGISGERIWVELKKILVGNHVNHL IHLIYDLDVAPYIGLPANASLEEFD KVSKNVDGFSPKPVTLLASLFKVQD DVTKLDLRLKIAKEEKNLGLFIVKN RKDLIKATDSSDPLKPYQDFIIDSR EPDATTRVCELLKYQGEHCLLKEMQ QWSIPPFPVSGHDIRKVGISSGKEI GALLQQLREQWKKSGYQMEKDELLS YIKKTTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TRNT1 tRNA nucleotidyl transferase 1 [ Homo sapiens (human) ]
• Official Symbol: TRNT1
• Synonyms: TRNT1; tRNA nucleotidyl transferase, CCA-adding, 1; CCA tRNA nucleotidyltransferase 1, mitochondrial; CCA1; CGI 47; MtCCA; mt CCA-adding enzyme; mt tRNA CCA-diphosphorylase; mt tRNA adenylyltransferase; mt tRNA CCA-pyrophosphorylase; tRNA-nucleotidyltransferase 1, mitochondrial; mitochondrial CCA-adding tRNA-nucleotidyltransferase; mitochondrial tRNA nucleotidyl transferase, CCA-adding; CGI-47
• Gene ID: 51095
• mRNA Refseq: NM_016000
• Protein Refseq: NP_057084
• MIM: 612907
• UniProt ID: Q96Q11