Recombinant Human TTL Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TTL-5778H
• Product Overview: TTL MS Standard C13 and N15-labeled recombinant protein (NP_714923) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin. Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination.
• Molecular Mass: 43.2 kDa
• AA Sequence: MYTFVVRDENSSVYAEVSRLLLATGHWKRLRRDNPRFNLMLGERNRLPFGRLGHEPGLVQLVNYYRGADKLCRKASLVKLIKTSPELAESCTWFPESYVIYPTNLKTPVAPAQNGIQPPISNSRTDEREFFLASYNRKKEDGEGNVWIAKSSAGAKGEGILISSEASELLDFIDNQGQVHVIQKYLEHPLLLEPGHRKFDIRSWVLVDHQYNIYLYREGVLRTASEPYHVDNFQDKTCHLTNHCIQKEYSKNYGKYEEGNEMFFKEFNQYLTSALNITLESSILLQIKHIIRNCLLSVEPAISTKHLPYQSFQLFGFDFMVDEELKVWLIEVNGAPACAQKLYAELCQGIVDIAISSVFPPPDVEQPQTQPAAFIKLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TTL tubulin tyrosine ligase [ Homo sapiens (human) ]
• Official Symbol: TTL
• Synonyms: TTL; tubulin tyrosine ligase; tubulin--tyrosine ligase; MGC46235; 2410003M22Rik; tubulin-tyrosine ligase
• Gene ID: 150465
• mRNA Refseq: NM_153712
• Protein Refseq: NP_714923
• MIM: 608291
• UniProt ID: Q8NG68