| Description : |
This gene encodes a component of the gamma-tubulin ring complex, which is required for microtubule nucleation. In mammalian cells, the protein localizes to centrosomes in association with gamma-tubulin. Crystal structure analysis revealed a structure composed of five helical bundles arranged around conserved hydrophobic cores. An exposed surface area located in the C-terminal domain is essential and sufficient for direct binding to gamma-tubulin. Mutations in this gene that alter microtubule organization are associated with microcephaly and chorioretinopathy. Alternative splicing results in multiple transcript variants. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
76 kDa |
| AA Sequence : |
MIHELLLALSGYPGSIFTWNKRSGL QVSQDFPFLHPSETSVLNRLCRLGT DYIRFTEFIEQYTGHVQQQDHHPSQ QGQGGLHGIYLRAFCTGLDSVLQPY RQALLDLEQEFLGDPHLSISHVNYF LDQFQLLFPSVMVVVEQIKSQKIHG CQILETVYKHSCGGLPPVRSALEKI LAVCHGVMYKQLSAWMLHGLLLDQH EEFFIKQGPSSGNVSAQPEEDEEDL GIGGLTGKQLRELQDLRLIEEENML APSLKQFSLRVEILPSYIPVRVAEK ILFVGESVQMFENQNVNLTRKGSIL KNQEDTFAAELHRLKQQPLFSLVDF EQVVDRIRSTVAEHLWKLMVEESDL LGQLKIIKDFYLLGRGELFQAFIDT AQHMLKTPPTAVTEHDVNVAFQQSA HKVLLDDDNLLPLLHLTIEYHGKEH KDATQAREGPSRETSPREAPASGWA ALGLSYKVQWPLHILFTPAVLEKYN VVFKYLLSVRRVQAELQHCWALQMQ RKHLKSNQTDAIKWRLRNHMAFLVD NLQYYLQVDVLESQFSQLLHQINST RDFESIRLAHDHFLSNLLAQSFILL KPVFHCLNEILDLCHSFCSLVSQNL GPLDERGAAQLSILVKGFSRQSSLL FKILSSVRNHQINSDLAQLLLRLDY NKYYTQAGGTLGSFGMTRTRPLEQK LISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
