Recombinant Human Tumor Necrosis Factor Receptor Superfamily, Member 11b, His-tagged

• Cat.No.: TNFRSF11B-5142H
• Product Overview: Recombinant human osteoprotegerin protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography techniques

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: Osteoprotegerin (OPG) is a member of the tumor necrosis factor (TNF)-related family, is referred to as TNFRSF11B and part of the OPG/receptor activator of NF-kB ligand (RANKL)/receptor activator of NF-kB (RANK) triad. This cytokine that lacks any apparent cell-association motifs and exists as a soluble secreted protein, network regulates the differentiation and activation of osteoclasts and hence the critical balance between bone formation (osteoblasts) and bone resorption (osteoclasts).
• Concentration: 0.5 mg/ml
• Form: Supplied as a liquid in PBS, pH 7.4, containing 10% glycerol.
• Purity: > 85 % by SDS - PAGE
• Sequence: 22-401 amino acids: ADPETFPPKY LHYDEETSHQ LLCDKCPPGT YLKQHCTAKW KTVCAPCPDH YYTDSWHTSD ECLYCSPVCK ELQYVKQECN RTHNRVCECK EGRYLEIEFC LKHRSCPPGF GVVQAGTPER NTVCKRCPDG FFSNETSSKA PCRKHTNCSV FGLLLTQKGN ATHDNICSGN SESTQKCGID VTLCEEAFFR FAVPTKFTPN WLSVLVDNLP GTK
• Molecular Mass: 45.6 kDa (398 aa) (On SDS-PAGE under denatured condition, apparent molecular weight of glycosylated rhOPG protein will be appeared at approximately 55 kDa)
• Applications: SDS-PAGE
• Endotoxin Levels: < 1.0 EU per ug of protein (determined by LAL method)
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: TNFRSF11B tumor necrosis factor receptor superfamily, member 11b [ Homo sapiens ]
• Official Symbol: TNFRSF11B
• Synonyms: TNFRSF11B; OPG; OCIF; Osteoclastogenesis inhibitory factor; TR1; MGC29565; Osteoprotegerin; Osteoclastogenesis inhibitory factor; tumor necrosis factor receptor superfamily, member 11b
• Gene ID: 4982
• mRNA Refseq: NM_002546
• Protein Refseq: NP_002537
• MIM: 602643
• UniProt ID: O00300
• Chromosome Location: 8q24
• Pathway: Cytokine-cytokine receptor interaction; Monoamine Transport; Osteoblast Signaling; Osteoclast differentiation
• Function: Binding; cytokine activity; receptor activity