Recombinant Human Tyrosine Kinase, Non-receptor, 2, GST-tagged, Active

• Cat.No.: ACK-234H
• Product Overview: Recombinant human ACK (110-476) was expressed by baculovirus inSf9 insect cellsusing an N-terminal GST tag. MW=66 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 110-476 a.a.
• Description: ACK is a tyrosine kinase that binds CDC42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of CDC42Hs. Overexpression of ACK in cancer cell lines of epithelial origin increases cellular motility and invasiveness. In a mouse model, ACK overexpression enhances the ability of a human breast cancer cell line to metastasize to the lung and increased mortality. Ligand stimulation of alpha-3 beta-1 integrin leads to activation of ACK which then enhances p130CAS phosphorylation and activation of RAC.
• Sequence: 110-476.
• Applications: Kinase Assay, Western Blot.
• Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: TNK2 tyrosine kinase, non-receptor, 2 [ Homo sapiens ]
• Synonyms: TNK2; tyrosine kinase, non-receptor, 2; ACK; ACK1; FLJ44758; FLJ45547; p21cdc42Hs; activated p21cdc42Hs kinase; activated Cdc42-associated kinase 1; EC 2.7.10.2
• Gene ID: 10188
• mRNA Refseq: NM_001010938
• Protein Refseq: NP_001010938
• MIM: 606994
• UniProt ID: Q07912
• Chromosome Location: 3q29
• Function: ATP binding; GTPase inhibitor activity; non-membrane spanning protein tyrosine kinase activity; magnesium ion binding; protein binding; transferase activity