Recombinant Human TYW3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TYW3-1881H
• Product Overview: TYW3 MS Standard C13 and N15-labeled recombinant protein (NP_612476) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW3 is the human homolog of a yeast gene essential for yW synthesis.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 29.8 kDa
• AA Sequence: MDRSAEFRKWKAQCLSKADLSRKGS VDEDVVELVQFLNMRDQFFTTSSCA GRILLLDRGINGFEVQKQNCCWLLV THKLCVKDDVIVALKKANGDATLKF EPFVLHVQCRQLQDAQILHSMAIDS GFRNSGITVGKRGKTMLVVRSTHGL EVPLSHKGKLMVTEEYIDFLLNVAN QKMEENKKRIERFYNCLQHALERET MTNLHPKIKEKNNSSYIHKKKRNPE KTRAQCITKESDEELENDDDDDLGI NVTIFPEDYTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TYW3 tRNA-yW synthesizing protein 3 homolog [ Homo sapiens (human) ]
• Official Symbol: TYW3
• Synonyms: TYW3; tRNA-yW synthesizing protein 3 homolog (S. cerevisiae); C1orf171, chromosome 1 open reading frame 171; tRNA wybutosine-synthesizing protein 3 homolog; FLJ40918; tRNA-yW-synthesizing protein 3; C1orf171
• Gene ID: 127253
• mRNA Refseq: NM_138467
• Protein Refseq: NP_612476
• MIM: 611245
• UniProt ID: Q6IPR3