Recombinant Human U2AF1 293 Cell Lysate

• Cat.No.: U2AF1-609HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for U2 small nuclear RNA auxiliary factor 1 (U2AF1), transcript variant a is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: U2AF1 U2 small nuclear RNA auxiliary factor 1 [ Homo sapiens ]
• Official Symbol: U2AF1
• Synonyms: U2AF1; U2 small nuclear RNA auxiliary factor 1; U2(RNU2) small nuclear RNA auxiliary factor 1 , U2(RNU2) small nuclear RNA auxiliary factor binding protein , U2AFBP; splicing factor U2AF 35 kDa subunit; RN; RNU2AF1; U2AF35; splicing factor U2AF 35kDa subunit; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit; FP793; U2AFBP; DKFZp313J1712
• Gene ID: 7307
• mRNA Refseq: NM_001025203
• Protein Refseq: NP_001020374
• MIM: 191317
• UniProt ID: Q01081
• Chromosome Location: 21q22.3
• Pathway: Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem; Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; RNA Polymerase II Transcription, organism-specific biosystem; RNA Polymerase II Transcription Termination, organism-specific biosystem; Shigellosis, organism-specific biosystem; Spliceosome, organism-specific biosystem