Recombinant Human UBA1 protein(Ser2-Arg1058), His&GST-tagged

• Cat.No.: UBA1-7333H
• Product Overview: Recombinant Human UBA1 (NP_003325.2) (Ser 2-Arg 1058) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His&GST
• Protein length: Ser2-Arg1058
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4, 10% gly, 0.5mM GSH Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human UBA1/GST chimera consists of 1294 amino acids and has a calculated molecular mass of 146 KDa. It migrates as an approximately 130 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: UBA1 ubiquitin-like modifier activating enzyme 1 [ Homo sapiens ]
• Official Symbol: UBA1
• Synonyms: UBA1; ubiquitin-like modifier activating enzyme 1; A1S9T, GXP1, UBE1, ubiquitin activating enzyme E1 , ubiquitin activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing); ubiquitin-like modifier-activating enzyme 1; POC20; POC20 centriolar protein homolog (Chlamydomonas); ubiquitin activating enzyme E1 homolog (yeast); UBE1X; POC20 centriolar protein homolog; UBA1, ubiquitin-activating enzyme E1 homolog A; A1S9T and BN75 temperature sensitivity complementing; A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; SMAX2; UBA1A; MGC4781
• Gene ID: 7317
• mRNA Refseq: NM_003334
• Protein Refseq: NP_003325
• MIM: 314370
• UniProt ID: P22314

Reviews

07/02/2022

The UBA1 protein comes highly recommended for its outstanding performance in various experimental applications.

07/07/2019

In ELISA assays, it has consistently exhibited exceptional reliability and accuracy, providing precise and reproducible results.

05/13/2017

Their prompt and knowledgeable guidance has been instrumental in effectively addressing my queries and optimizing the utilization of the UBA1 protein in my studies.

Q&As

Are there any clinical trials involving UBA1 as a target?

Some early-phase clinical trials are exploring UBA1 as a potential target in the context of ALS and cancer.

How can UBA1 be targeted for therapeutic purposes?

Targeting UBA1 with small molecules or gene therapy may provide a potential avenue for treating UBA1-related diseases.

How does UBA1 function in the cell?

UBA1 activates ubiquitin, a small protein that tags other proteins for degradation by the proteasome.

What diseases are associated with UBA1 dysfunction?

UBA1 dysfunction has been linked to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and certain types of cancer.

Can UBA1 be a diagnostic marker for ALS?

Research suggests that altered UBA1 levels in the cerebrospinal fluid may be a potential biomarker for ALS diagnosis.