Recombinant Human UBA2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: UBA2-4965H
• Product Overview: UBA2 MS Standard C13 and N15-labeled recombinant protein (NP_005490) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 71 kDa
• AA Sequence: MALSRGLPRELAEAVAGGRVLVVGA GGIGCELLKNLVLTGFSHIDLIDLD TIDVSNLNRQFLFQKKHVGRSKAQV AKESVLQFYPKANIVAYHDSIMNPD YNVEFFRQFILVMNALDNRAARNHV NRMCLAADVPLIESGTAGYLGQVTT IKKGVTECYECHPKPTQRTFPGCTI RNTPSEPIHCIVWAKYLFNQLFGEE DADQEVSPDRADPEAAWEPTEAEAR ARASNEDGDIKRISTKEWAKSTGYD PVKLFTKLFKDDIRYLLTMDKLWRK RKPPVPLDWAEVQSQGEETNASDQQ NEPQLGLKDQQVLDVKSYARLFSKS IETLRVHLAEKGDGAELIWDKDDPS AMDFVTSAANLRMHIFSMNMKSRFD IKSMAGNIIPAIATTNAVIAGLIVL EGLKILSGKIDQCRTIFLNKQPNPR KKLLVPCALDPPNPNCYVCASKPEV TVRLNVHKVTVLTLQDKIVKEKFAM VAPDVQIEDGKGTILISSEEGETEA NNHKKLSEFGIRNGSRLQADDFLQD YTLLINILHSEDLGKDVEFEVVGDA PEKVGPKQAEDAAKSITNGSDDGAQ PSTSTAQEQDDVLIVDSDEEDSSNN ADVSEEERSRKRKLDEKENLSAKRS RIEQKEELDDVIALDTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: UBA2 ubiquitin-like modifier activating enzyme 2 [ Homo sapiens (human) ]
• Official Symbol: UBA2
• Synonyms: UBA2; ubiquitin-like modifier activating enzyme 2; SAE2, SUMO1 activating enzyme subunit 2; SUMO-activating enzyme subunit 2; ARX; FLJ13058; HRIHFB2115; ubiquitin activating enzyme E1 homolog (yeast); SUMO1 activating enzyme subunit 2; SUMO-1 activating enzyme subunit 2; ubiquitin-like 1-activating enzyme E1B; anthracycline-associated resistance ARX; UBA2, ubiquitin-activating enzyme E1 homolog; SAE2
• Gene ID: 10054
• mRNA Refseq: NM_005499
• Protein Refseq: NP_005490
• MIM: 613295
• UniProt ID: Q9UBT2