Recombinant Human UBA7 protein, His & T7-tagged

• Cat.No.: UBA7-5755H
• Product Overview: Recombinant Human UBA7 aa. (Lys429~Val488 (Accession # P41226)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Lys429~Val488 (Accession # P41226)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: UBA7 ubiquitin like modifier activating enzyme 7 [ Homo sapiens (human) ]
• Official Symbol: UBA7
• Synonyms: UBA7; ubiquitin like modifier activating enzyme 7; D8; UBE2; UBA1B; UBE1L; ubiquitin-like modifier-activating enzyme 7; UBA1, ubiquitin-activating enzyme E1 homolog B; UBA7, ubiquitin-activating enzyme E1; ubiquitin-activating enzyme 7; ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1-related protein; ubiquitin-activating enzyme-2
• Gene ID: 7318
• mRNA Refseq: NM_003335.2
• Protein Refseq: NP_003326.2
• UniProt ID: P41226