"UBB" Related Products


Recombinant Human UBB protein

Cat.No.: UBB-572H
Product Overview: Recombinant Human UBB was expressed in bacterial.
Source: E. coli
Species: Human
Form: X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl
Bio-activity: Di-Ub (K48-linked) FRET TAMRA Pos1 is ideal for use as a substrate for Ubiquitin-specific hydrolases with activity toward K48 linkages. We recommend an assay buffer consisting of 50 mM Hepes pH 7.5, 150 mM NaCl, 2 mM DTT. Fluorescence can be monitored with an excitation wavelength of 544 nM and an emission wavelength of 572 nM. Reaction conditions will need to be optimized for each specific application. We recommend an initial Di-Ub (K48-linked) FRET TAMRA Pos1 concentration of 100-500 nM.
Molecular Mass: Predicted Molecular Mass: 17 kDa
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Storage: Protect from light and avoid repeated freeze-thaw cycles.12 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.
Gene Name: UBB ubiquitin B [ Homo sapiens ]
Official Symbol: UBB
Synonyms: UBB; ubiquitin B; polyubiquitin-B; FLJ25987; MGC8385; polyubiquitin B; UBC; UBA52; RPS27A;
Gene ID: 7314
mRNA Refseq: NM_018955
Protein Refseq: NP_061828
MIM: 191339
UniProt ID: P0CG47
Chromosome Location: 17p12-p11.2
Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Cyclin B, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem
Function: protein binding; ubiquitin-protein ligase activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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