"UBB" Related Products

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Recombinant Human UBB protein

Cat.No.: UBB-209H
Product Overview: Recombinant Human UBB was expressed in E. coli.
Description: Linkage specific poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB's") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules. K63-linked tetra-Ubiquitin chains are manufactured using recombinant wild-type human Ubiquitin and linkage-specific enzymes. The use of purely enzymatic techniques avoids the potential for contaminating synthetic intermediates. This fluorogenic substrate is intended for use with deubiquitinating enzymes that are unable to use mono-Ubiquitin-Rh110 as a substrate, or enzymes with activity that is stimulated by poly-Ubiquitin chains. The substrate consists of a K63-linked tetra-Ubiquitin chain with a single rhodamine attached to the C-terminus of the proximal Ubiquitin.
Source: E. coli
Species: Human
Form: X mg/ml (X μM) in 50 mM HEPES pH 7.0, 50 mM NaCl
Bio-activity: K63-linked tetra-Ubiquitin Rhodamine 110 is ideal for use in assays requiring fluorescent detection of deubiquitinase activity. Optimal fluorescence at pH 8.0 is monitored with excitation and emission wavelengths of 485 nm and 535 nm, respectively. Reaction conditions will need to optimized for each specific application. We recommend an initial concentration of 0.1-1 μM.
Molecular Mass: Predicted Molecular Mass: 35 kDa
Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
Gene Name: UBB ubiquitin B [ Homo sapiens ]
Official Symbol: UBB
Synonyms: UBB; ubiquitin B; polyubiquitin-B; FLJ25987; MGC8385; polyubiquitin B; UBC; UBA52; RPS27A;
Gene ID: 7314
mRNA Refseq: NM_018955
Protein Refseq: NP_061828
MIM: 191339
UniProt ID: P0CG47

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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