| Cat.No. : |
UBE2D3-2004H |
| Product Overview : |
Recombinant Human UBE2D3 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Met147) of human UBE2D3 (Accession #NP_003331.1) fused with a 6×His tag at the C-terminus. |
| Description : |
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His |
| Form : |
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5 % mannitol. |
| Protein length : |
Met1-Met147 |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : |
> 90% as determined by SDS-PAGE |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70ºC. |
| Storage : |
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
