Recombinant Human UBE2H Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: UBE2H-1325H
• Product Overview: UBE2H MS Standard C13 and N15-labeled recombinant protein (NP_003335) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 20.7 kDa
• AA Sequence: MSSPSPGKRRMDTDVVKLIESKHEV TILGGLNEFVVKFYGPQGTPYEGGV WKVRVDLPDKYPFKSPSIGFMNKIF HPNIDEASGTVCLDVINQTWTALYD LTNIFESFLPQLLAYPNPIDPLNGD AAAMYLHRPEEYKQKIKEYIQKYAT EEALKEQEEGTGDSSSESSMSDFSE DEAQDMELTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: UBE2H ubiquitin-conjugating enzyme E2H [ Homo sapiens (human) ]
• Official Symbol: UBE2H
• Synonyms: UBE2H; ubiquitin-conjugating enzyme E2H; ubiquitin conjugating enzyme E2H (homologous to yeast UBC8), ubiquitin conjugating enzyme E2H (UBC8 homolog, yeast); ubiquitin-conjugating enzyme E2 H; UBC8; UBCH; ubiquitin-protein ligase H; ubiquitin carrier protein H; ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast); ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8); UBCH2; E2-20K
• Gene ID: 7328
• mRNA Refseq: NM_003344
• Protein Refseq: NP_003335
• MIM: 601082
• UniProt ID: P62256