Recombinant Human UBE2K protein, GST-tagged

• Cat.No.: UBE2K-3537H
• Product Overview: Recombinant Human UBE2K protein(1-200 aa), fused with N-terminal GST tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1-200 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 8.0). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 100 mM reduced glutathione (GSH).
• AASequence: MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: UBE2K ubiquitin-conjugating enzyme E2K [ Homo sapiens ]
• Official Symbol: UBE2K
• Synonyms: UBE2K; ubiquitin-conjugating enzyme E2K; HIP2, huntingtin interacting protein 2 , ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast); ubiquitin-conjugating enzyme E2 K; HYPG; UBC1; HIP-2; E2(25K); ubiquitin-protein ligase; ubiquitin carrier protein; huntingtin interacting protein 2; huntingtin-interacting protein 2; ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2(25K); ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); LIG; HIP2; E2-25K; DKFZp564C1216; DKFZp686J24237
• Gene ID: 3093
• mRNA Refseq: NM_001111112
• Protein Refseq: NP_001104582
• MIM: 602846
• UniProt ID: P61086