Recombinant Human Ubiquitin-conjugating Enzyme E2I

• Cat.No.: UBE2I-34H
• Product Overview: Recombinant Human Ubiquitin-conjugating enzyme E2I was expressed inE.coli. MW =18 kDa (158 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Human Ubc9 is homologous to ubiquitin-conjugating enzymes (E2s). However, instead of conjugating ubiquitin, it conjugates an ubiquitin homologue, small ubiquitin-like modifier 1(SUMO-1). And hUbc9 retains striking structural and functional conservation with yeast Ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin requires the activities of ubiquitin-activating (E1) and –conjugating (E2) enzymes.
• Amino Acid Sequence: MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS
• Form: Liquid. In 50 mM HEPES (pH 7.5) containing 1 mM DTT, 150 mM NaCl 10% glycerol.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UBE2Iubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) [Homo sapiens]
• Synonyms: P18; UBC9; C358B7.1; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); UBC9_HUMAN; SUMO-conjugating enzyme UBC9; EC 6.3.2.-; SUMO-protein ligase; Ubiquitin-protein ligase I; Ubiquitin carrier protein I; Ubiquitin carrier protein 9; UBE2I;UBCE9; Ubiquitin-protein ligase I; ubiquitin carrier protein; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme E2I; ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme
• Gene ID: 7329
• mRNA Refseq: NM_003345
• Protein Refseq: NP_003336
• MIM: 601661
• UniProt ID: P63279
• Chromosome Location: 16p13.3
• Function: HLH domain binding;ligase activity;protein binding; small conjugating protein ligase activity