Recombinant Human V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog (avian), His-tagged

• Cat.No.: ERBB2-1215H
• Product Overview: Recombinant humanERBB2 cytoplasmic domain (aa 676-1255) with His tag. No special measures weretaken to activate this kinase.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 676-1255 a.a.
• Description: ERBB2 stands for"Human Epidermal growth factor Receptor 2" and is a protein givinghigher aggressiveness in breast cancers.It is a member of the ErbB protein family, more commonly knownas the epidermal growth factor receptor family. HER2/neu has also beendesignated as CD340 (cluster ofdifferentiation 340) and p185. It is encoded by the ERBB2 gene.
• Form: Liquid in 50 mMTris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.01% CHAPS + 2 Mm DTT + 50%glycerol.
• Molecular Weight: 68.6 kDa
• Storage: Stablefor 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: ERBB2

Gene Information

• Gene Name: ERBB2 v-erb-b2 erythroblasticleukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogenehomolog (avian) [ Homo sapiens ]
• Synonyms: ERBB2; v-erb-b2erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derivedoncogene homolog (avian); NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19;HER-2/neu; receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogeneNeu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph nodegene 19 protein; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastomaderived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viraloncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
• Gene ID: 2064
• mRNA Refseq: NM_004448
• Protein Refseq: NP_004439
• MIM: 164870
• UniProt ID: P04626
• Chromosome Location: 17q11.2-q12
• Pathway: Adherens junction;Alpha6-Beta4 Integrin Signaling Pathway; Axon guidance; Bladder cancer; Calciumsignaling pathway; Developmental Biology; Endometrial cancer; ErbB receptorsignaling network; ErbB signaling pathway; ErbB2/ErbB3 signaling events;Focal Adhesion; Focal adhesion; Non-small cell lung cancer; Pancreaticcancer; Sema4D in semaphorin signaling
• Function: ATP binding; ErbB-3class receptor binding; Hsp90 protein binding; RNA polymerase I core binding;epidermal growth factor-activated receptor activity; glycoprotein binding;contributes_to growth factor binding; identical protein binding; nucleotidebinding; protein C-terminus binding; protein binding; protein dimerizationactivity; protein heterodimerization activity; protein phosphatase binding;receptor activity

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.