Recombinant Human V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog (avian), Methionine

• Cat.No.: ERBB2-894H
• Product Overview: ErbB-2 Human Recombinant is a 43.4 kDa protein containing 397 amino acid residues of the human Herstatin, and an extra Methionine at N-Termainal (underlined), produced in E.coli. The ErbB2 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: ERBB2-894H
• Description: HER-2/neu (erbB-2) encodes an 185-kDa orphan receptor tyrosine kinase that is constitutively active as a dimer and displays potent oncogenic activity when overexpressed. Herstatin, as the product of alternative HER-2 transcript, retains intron 8.
• Source: Human
• Host: Escherichia Coli
• Form: Filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH=4.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to add 0.1M Acetate buffer pH=4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10mg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. It is recommended to add 5mM DTT and 0.1-0.15M NaCl before freezing in order to prevent potential aggregation.
• Amino acid sequence: MTQVCTGTDM KLRLPASPET HLDMLRHLYQ GCQVVQGNLE LTYLPTNASL SFLQDIQEVQ GYVLIAHNQV RQVPLQRLRI VRGTQLFEDN YALAVLDNGD PLNNTTPVTG ASPGGLRELQ LRSLTEILKG GVLIQRNPQL CYQDTILWKD IFHKNNQLAL TLIDTNRSRA CHPCSPMCKG SRCWGESSED CQSLTRTVCA GGCARCKGPL PTDCCHEQCA AGCTGPKHSD CLACLHFNHS GICELHCPAL VTYNTDTFES MPNPEGRYTF GASCVTACPY NYLSTDVGSC TLVCPLHNQE VTAEDGTQRC EKCSKPCARG THSLPPRPAA VPVPLRMQPG PAHPVLSFLR PSWDLVSAFY SLPLAPLSPT SVPISPVSVG RGPDPDAHVA VDLSRYEG.
• Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time. The lyophilized protein remains stable until the expiry date when stored at -20°C.

Gene Information

• Gene Name: ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Homo sapiens ]
• Official Symbol: ERBB2
• Synonyms: ERBB2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); NEU; NGL; HER2; TKR1; CD340; HER-2; HER-2/neu; erbB-2; herstatin; c-erb B2/neu protein; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); C-erbB-2; EC 2.7.10.1; p185erbB2; CD340 antigen; MLN 19; NEU proto-oncogene; Tyrosine kinase-type cell surface receptor HER2
• Gene ID: 2064
• mRNA Refseq: NM_001005862
• Protein Refseq: NP_001005862
• MIM: 164870
• UniProt ID: P04626
• Chromosome Location: 17q11.2-q12; 17q21.1
• Pathway: Adherens junction; Bladder cancer; Calcium signaling pathway; Endometrial cancer
• Function: ATP binding; ErbB-3 class receptor binding; Hsp90 protein binding; epidermal growth factor receptor activity; glycoprotein binding; growth factor binding; identical protein binding; nucleotide binding; protein C-terminus binding; protein heterodimerization activity; protein phosphatase binding; receptor signaling protein tyrosine kinase activity; transferase activity; transmembrane receptor activity; ubiquitin protein ligase binding