Active Recombinant Human VEGFA protein

• Cat.No.: VEGFA-709H
• Product Overview: Active Recombinant Human VEGFA protein(P15692-4) (Met1-Arg191) was expressed in Insect Cells. Human and Cynomolgus VEGF165 sequences are identical.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Protein Length: 1-191 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 3-5 ng/ml.
• Molecular Mass: The recombinant human VEGF consists of 165 amino acids after removal of the signal peptide and predicts a molecular mass of 19.2 kDa. Due to different glycosylation, the apparent molecular mass of rhVEGF165 is approximately 20 and 22 kDa in SDS-PAGE under reducing conditions, corresponding to the monomer.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001020537.2
• Unit ID: P15692
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/P15692

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor, VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• protein Refseq: NP_001020537