Active Recombinant Human VEGF165 Protein, Low Endotoxin

• Cat.No.: VEGF165-1004H
• Product Overview: Active Recombinant Human VEGF165 Protein(P15692-4)(Ala27-Arg191) is expressed from HEK293 without tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: 27-191 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50 for this effect is < 10 ng/mL.
• Molecular Mass: The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.