Active Recombinant Human VEGFA Protein, His & Avi-tagged, Biotinylated

• Cat.No.: VEGFA-273H
• Product Overview: Biotinylated Recombinant Human VEGFA Protein (Ala 27 - Arg 191) (P15692-4), fused with a polyhistidine tag at the N-terminus, followed by an Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His & Avi
• Predicted N Terminal: His
• Form: Lyophilized from sterile PBS, pH7.4 , 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human VEGFR2/R3, Fc tag at 2μg/mL (100 μL/well) can bind Biotinylated Human VEGF165 with a linear range of 0.5-7.8 ng/mL. Immobilized Anti-VEGF MAb,Human IgG1 (Avastin) at 5μg/mL (100 μL/well) can bind Biotinylated Human VEGF165 with a linear range of 1-15.6 ng/mL.
• Molecular Mass: Recombinant Human VEGFA Protein has a calculated MW of 22.4 kDa (monomer). As a result of glycosylation, The protein migrates as 30 kDa (monomer) under reducing (R) condition and 48-60 kDa (homodimer) under non-reducing (NR) condition on SDS-PAGE gel.
• Protein length: Ala 27 - Arg 191
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• UniProt ID: P15692
• Chromosome Location: 6p12
• Pathway: Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endochondral Ossification, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem
• Function: cell surface binding; chemoattractant activity; cytokine activity; cytokine activity; extracellular matrix binding; fibronectin binding; growth factor activity; growth factor activity; heparin binding; heparin binding; platelet-derived growth factor receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; receptor agonist activity; vascular endothelial growth factor receptor 1 binding; vascular endothelial growth factor receptor 2 binding; vascular endothelial growth factor receptor binding