Recombinant Human VEGFB, His-tagged

• Cat.No.: VEGFB-554H
• Product Overview: Recombinant VEGF-B protein was expressed in E.coli and purified by using conventional chromatography techniques (22-207 aa).

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: VEGF-B, also known as Vascular endothelial growth factor-B, is one of 5 mammalian VEGF family members, the others being VEGF-A, VEGF-C, VEGF-D, and placental growth factor (PlGF). VEGF-B binds to one of the VEGF receptor tyrosine kinases, namely VEGF receptor-1(VEGFR1, Flt1), but not to VEGFR2 and VEGFR3. VEGF-B is homodimeric, heparin-binding glycoprotein involved in embryonic angiogenesis and vasculogenesis.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol
• Molecular Weight: 21.6 kDa (207aa), confirmed by MALDI-TOF. (molecular weight on SDS-PAGE will appear higher)
• Purity: > 90% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Endotoxin Level: < 1.0 EU per 1μg of protein (determined by LAL method)
• Biological activity: Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml.
• Activity Assay: 1. Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast ) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay medium: serum free RPMI 1640 4. Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul) 5. Incubation time : 24 hr (after sample treatment) 6. Concentration range: 10pg/ml - 10ug/ml 7. Detection method : BrdU assay
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles

Gene Information

• Gene Name: VEGFB vascular endothelial growth factor B [ Homo sapiens ]
• Official Symbol: VEGFB
• Synonyms: VEGFB; VEGF-B; VEGFL; VRF; VEGF-related factor; vascular endothelial growth factor B; VEGF-related factor (VRF)
• Gene ID: 7423
• mRNA Refseq: NM_003377
• Protein Refseq: NP_003368
• MIM: 601398
• UniProt ID: P49765
• Chromosome Location: 11q13
• Pathway: Cytokine-cytokine receptor interaction; Focal adhesion; Pancreatic cancer; Renal cell carcinoma; mTOR signaling pathway; Hemostasis; Signaling by VEGF
• Function: growth factor activity; heparin binding; protein binding; vascular endothelial growth factor receptor binding