Recombinant Human VLDLR protein, His-tagged

• Cat.No.: VLDLR-892H
• Product Overview: Recombinant Human VLDLR protein(P98155-1)(Gly28-Ser797), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gly28-Ser797
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Human VLDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.72 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 85.9 kDa. Due to glycosylation, the protein migrates to 115-125 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VLDLR very low density lipoprotein receptor [ Homo sapiens ]
• Official Symbol: VLDLR
• Synonyms: VLDLR; very low density lipoprotein receptor; very low-density lipoprotein receptor; VLDL-R; VLDL receptor; CARMQ1; CHRMQ1; VLDLRCH; FLJ35024
• Gene ID: 7436
• mRNA Refseq: NM_001018056
• Protein Refseq: NP_001018066
• MIM: 192977
• UniProt ID: P98155