Recombinant Human VRK1 Protein, His & GST-tagged

• Cat.No.: VRK1-322H
• Product Overview: Recombinant human VRK1 (Q99986) (Met 1-Lys 396) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-396 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 5%~8% trehalose and mannitol.
• Bio-activity: No Kinase Activity
• Molecular Mass: The recombinant human VRK1/GST chimera consists of 633 amino acids and has a calculated molecular mass of 73 KDa. It migrates as an approximately 65-70 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >88 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: VRK1 vaccinia related kinase 1 [ Homo sapiens ]
• Official Symbol: VRK1
• Synonyms: VRK1; vaccinia related kinase 1; serine/threonine-protein kinase VRK1; vaccinia-related kinase 1; vaccinia virus B1R-related kinase 1; PCH1; MGC117401; MGC138280; MGC142070
• Gene ID: 7443
• mRNA Refseq: NM_003384
• Protein Refseq: NP_003375
• MIM: 602168
• UniProt ID: Q99986
• Chromosome Location: 14q32