"VRK1" Related Products

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Recombinant Human VRK1 Protein, His & GST-tagged

Cat.No.: VRK1-322H
Product Overview: Recombinant human VRK1 (Q99986) (Met 1-Lys 396) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source: Baculovirus-Insect Cells
Species: Human
Tag: His & GST
Predicted N Terminal: Met
Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 5%~8% trehalose and mannitol.
Bio-activity: No Kinase Activity
Molecular Mass: The recombinant human VRK1/GST chimera consists of 633 amino acids and has a calculated molecular mass of 73 KDa. It migrates as an approximately 65-70 KDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >88 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: VRK1 vaccinia related kinase 1 [ Homo sapiens ]
Official Symbol: VRK1
Synonyms: VRK1; vaccinia related kinase 1; serine/threonine-protein kinase VRK1; vaccinia-related kinase 1; vaccinia virus B1R-related kinase 1; PCH1; MGC117401; MGC138280; MGC142070;
Gene ID: 7443
mRNA Refseq: NM_003384
Protein Refseq: NP_003375
MIM: 602168
UniProt ID: Q99986
Chromosome Location: 14q32

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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