Recombinant Mouse VRK1 Protein (1-140 aa), His-Myc-tagged

• Cat.No.: VRK1-2229M
• Product Overview: Recombinant Mouse VRK1 Protein (1-140 aa) is produced by Yeast expression system. This protein is fused with a 10xHis tag at the N-terminal and a Myc tag at the C-terminal. Research Area: Cell Biology. Protein Description: Full Length.

Specification

• Description: Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.
• Source: Yeast
• Species: Mouse
• Tag: His-Myc
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 53.7 kDa
• Protein length: 1-140 aa
• AA Sequence: MPRVKAAQAGRPGPAKRRLAEQFAA GEVLTDMSRKEWKLGLPIGQGGFGC IYLADTNSSKPVGSDAPCVVKVEPS DNGPLFTELKFYQRAAKPEQIQKWI RTHKLKYLGVPKYWGSGLHDKNGKS YRFMIMDRFGSDLQKIYEANAKRFS RKTVLQLSLRILDILEYIHEHEYVH GDIKASNLLLSHKNPDQVYLVDYGL AYRYCPDGVHKEYKEDPKRCHDGTL EFTSIDAHKGVAPSRRGDLEILGYC MIQWLSGCLPWEDNLKDPNYVRDSK IRYRDNVAALMEKCFPEKNKPGEIA KYMESVKLLEYTEKPLYQNLRDILL QGLKAIGSKDDGKLDFSAVENGSVK TRPASKKRKKEAEESAVCAVEDMEC SDTQVQEAAQTRSVESQGAIHGSMS QPAAGCSSSDSSRRQQHLGLEQDML RLDRRGSRTRKKAQK
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: Vrk1 vaccinia related kinase 1 [ Mus musculus ]
• Official Symbol: VRK1
• Synonyms: VRK1; vaccinia related kinase 1; serine/threonine-protein kinase 51PK; 51PK
• Gene ID: 22367
• mRNA Refseq: NM_001029843
• Protein Refseq: NP_001025014
• UniProt ID: Q80X41