Recombinant Human VTCN1 protein, Fc-tagged, low endotoxin

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Recombinant Human VTCN1 protein, Fc-tagged, low endotoxin

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Cat.No. : VTCN1-572H
Product Overview : Recombinant Human VTCN1 (Phe29-Ala258) protein (low endotoxin) was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
Description : V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1/B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.
Source : HEK293
Species : Human
Tag : human IgG1 Fc
Predicted N Terminal : Phe29
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 51.8 kDa. The protein migrates as 66-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Phe29-Ala258
Endotoxin : Less than 0.01 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of VTCN1-572H :

Human B7-H4, Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of VTCN1-572H :

Immobilized Human B7-H4, Fc Tag, low endotoxin at 0.5 μg/mL (100 μL/well) can bind Anti-B7-H4 MAb (Human IgG1) with a linear range of 0.2-8 ng/mL (QC tested).

Gene Name : VTCN1
Official Symbol : VTCN1
Synonyms : VTCN1; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; B7 family member; H4; B7 superfamily member 1; B7 H4; B7H4; B7S1; B7X; FLJ22418; B7 family member, H4; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4
Gene ID : 79679
mRNA Refseq : NM_001253849
Protein Refseq : NP_001240778
MIM : 608162
UniProt ID : Q7Z7D3

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