| Source : |
HEK293 |
| Species : |
Canine |
| Tag : |
His |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Monoclonal Anti-Human B7-H4 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Canine B7-H4, His Tag with a linear range of 1-16 ng/mL.Immobilized Canine B7-H4, His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human B7-H4 Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL. |
| Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 27.2 kDa. The protein migrates as 44-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein Length : |
Phe 29-Ala 258 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE.>95% as determined by SEC-MALS. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
