Recombinant Human VTCN1 Protein, His & Avi-tagged, Biotinylated

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Recombinant Human VTCN1 Protein, His & Avi-tagged, Biotinylated

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Cat.No. : VTCN1-333H
Product Overview : Recombinant Human VTCN1 Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Ala 258 (Accession # NP_078902). Predicted N-terminus: Phe 29. This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 28 kDa. The protein migrates as 45-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source : HEK293
Species : Human
Tag : His & Avi
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Anti-B7-H4 mAb,Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human VTCN1 protein, His & Avi-tagged, Biotinylated with a linear range of 3-48 ng/mL (QC tested).
Molecular Mass : 28 kDa
Protein length : Phe 29 - Ala 258
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name : VTCN1 V-set domain containing T cell activation inhibitor 1 [ Homo sapiens ]
Official Symbol : VTCN1
Synonyms : VTCN1; B7 H4; B7H4; B7S1; B7X; FLJ22418; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4;
Gene ID : 79679
mRNA Refseq : NM_001253849
Protein Refseq : NP_001240778
MIM : 608162
UniProt ID : Q7Z7D3
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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