Recombinant Human VTN protein(Met1-Leu478), His-tagged

• Cat.No.: VTN-220H
• Product Overview: Recombinant Human VTN (NP_000629.3) (Met1-Leu478) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-478 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells.When cells are added to Vitronectin-coated plates (10 μg/mL and 100 μL/well), approximately >40% cells will adhere specifically after 30 minutes at 37°C.
• Molecular Mass: The recombinant human VTN consists of 470 amino acids and predicts a molecular mass of 53.7 kDa. As a result of glycosylation, it migrates as an approximately 68.1 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 10 EU per mg protein.
• Purity: ≥95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VTN vitronectin [ Homo sapiens ]
• Official Symbol: VTN
• Synonyms: VTN; vitronectin; vitronectin (serum spreading factor, somatomedin B, complement S protein); complement S protein; serum spreading factor; somatomedin B; VN; epibolin; S-protein; complement S-protein; serum-spreading factor; V75; VNT
• Gene ID: 7448
• mRNA Refseq: NM_000638
• Protein Refseq: NP_000629
• MIM: 193190
• UniProt ID: P04004