Recombinant Human VWF Protein, His-Avi-tagged, Biotinylated

• Cat.No.: VWF-894HB
• Product Overview: Biotinylated Recombinant Human VWF Protein(AAB59458.1) (Met1-Lys2813) was expressed in CHO with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Specification

• Species: Human
• Source: CHO
• Tag: Avi&His
• Protein Length: 1-2813 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant Human Von Willebrand Factor/vWF comprises 2817 amino acids and has a predicted molecular mass of 310.37 kDa. It migrates as an approximately 300.1 and 245.9 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VWF von Willebrand factor [ Homo sapiens ]
• Official Symbol: VWF
• Synonyms: VWF; von Willebrand factor; F8VWF; coagulation factor VIII VWF; VWD
• Gene ID: 7450
• mRNA Refseq: NM_000552
• Protein Refseq: NP_000543
• MIM: 613160
• UniProt ID: P04275