Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human WIF1 protein

Cat.No. : WIF1-577H
Product Overview : Recombinant Human Wnt Inhibitory Factor 1 is produced by our Mammalian expression system and the target gene encoding Gly29-Trp379 is expressed with a 6His tag at the C-terminus.
  • Specification
  • Gene Information
  • Related Products
Description : WIF (Wnt Inhibitory Factor) is a secreted protein that binds to Wnt proteins and inhibits their activity. The protein is synthesized as a 379 amino acid (AA) molecule that contains an N-terminal signal sequence, 150 AA WIF domain, 5 EGF-like repeats, and a hydrophilic domain at the carboxy terminus. In situ hybridization analysis from the frog, Xenopus laevis, and zebrafish indicate that the message is highly expressed in presomitic mesoderm, the notochord, anterior regions of the brain, branchial arches, nasal placodes, and otic vescicles. WIF inhibits secondary axis induction by Wnts and promotes secondary axis induction by Chordin in Xenopus embryos. In vitro, WIF binds to Drosophila Wingless and Xenopus Wnt8 proteins. WIF-1 is implicated as an early event tumor suppressor in cancers of the prostate, breast, lung and bladder. However, WIF-1’s role in carcinogenesis may not be that simple since in other cancer types, such as colon adenocarcinoma, WIF facilitates tumorigenesis. Human WIF-1 shares 94% and 82% amino acid identity with mouse and frog, respectively.
Source : HEK293
Species : Human
Form : Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NaAc, 150mM NaCl, 0.5% CHAPS, pH 4.0.
AA Sequence : GPPQEESLYLWIDAHQARVLIGFEE DILIVSEGKMAPFTHDFRKAQQRMP AIPVNIHSMNFTWQAAGQAEYFYEF LSLRSLDKGIMADPTVNVPLLGTVP HKASVVQVGFPCLGKQDGVAAFEVD VIVMNSEGNTILKTPQNAIFFKTCQ QAECPGGCRNGGFCNERRICECPDG FHGPHCEKALCTPRCMNGGLCVTPG FCICPPGFYGVNCDKANCSTTCFNG GTCFYPGKCICPPGLEGEQCEISKC PQPCRNGGKCIGKSKCKCSKGYQGD LCSKPVCEPGCGAHGTCHEPNKCQC QEGWHGRHCNKRYEASLIHALRPAG AQLRQHTPSLKKAEERRDPPESNYI WVDHHHHHH
Endotoxin : Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Purity : Greater than 95% as determined by reducing SDS-PAGE.
Storage : Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution : It is not recommended to reconstitute to a concentration less than 100 μg/ml
Gene Name : WIF1 WNT inhibitory factor 1 [ Homo sapiens ]
Official Symbol : WIF1
Synonyms : WIF1; WNT inhibitory factor 1; wnt inhibitory factor 1; WIF-1
Gene ID : 11197
mRNA Refseq : NM_007191
Protein Refseq : NP_009122
MIM : 605186
UniProt ID : Q9Y5W5

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (0)

Ask a question

Customer Reviews (0)

Write a review

Ask a Question for All WIF1 Products

Required fields are marked with *

My Review for All WIF1 Products

Required fields are marked with *

0

Inquiry Basket

cartIcon
logo

FOLLOW US

Terms and Conditions        Privacy Policy

Copyright © 2024 Creative BioMart. All Rights Reserved.

Contact Us

  • /

Stay Updated on the Latest Bioscience Trends