Recombinant Human WRN

• Cat.No.: WRN-30699TH
• Product Overview: Recombinant fragment of Human Werners syndrome helicase WRN with a proprietary tag: predicted molecular weight 37.84 kDa.

Specification

• Description: This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3 to 5 DNA helicase activity, and is also a 3 to 5 exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.
• Protein length: 111 amino acids
• Molecular Weight: 37.840kDa inclusive of tags
• Source: Wheat germ
• Biological activity: useful for Antibody Production and Protein Array
• Form: Liquid
• Purity: Proprietary Purification
• Storage buffer: pH: 8.00Constituents:0.79% Tris HCl, 0.31% GlutathioneNote: Glutathione is reduced
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: NPPVNSDMSKISLIRMLVPENIDTY LIHMAIEILKHGPDSGLQPSCDVNK RRCFPGSEEICSSSKRSKEEVGINT ETSSAERKRRLPVWFAKGSDTSKKL MDKTKRGGLFS
• Sequence Similarities: Belongs to the helicase family. RecQ subfamily.Contains 1 3-5 exonuclease domain.Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain.Contains 1 HRDC domain.

Gene Information

• Gene Name: WRN Werner syndrome, RecQ helicase-like [ Homo sapiens ]
• Official Symbol: WRN
• Synonyms: WRN; Werner syndrome, RecQ helicase-like; Werner syndrome; Werner syndrome ATP-dependent helicase; RECQ3; RECQL2
• Gene ID: 7486
• mRNA Refseq: NM_000553
• Protein Refseq: NP_000544
• MIM: 604611
• Uniprot ID: Q14191
• Chromosome Location: 8p12
• Pathway: Regulation of Telomerase, organism-specific biosystem
• Function: 3-5 DNA helicase activity; 3-5 exonuclease activity; ATP binding; ATP-dependent 3-5 DNA helicase activity; ATP-dependent DNA helicase activity