Recombinant Human X-ray repair complementing defective repair in Chinese hamster cells 2, His-tagged

• Cat.No.: XRCC2-412H
• Product Overview: XRCC2,1-280aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-280 a.a.
• Description: XRCC2 is a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the repair of DNA double-strand breaks by homologous recombination and it functionally complements Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents. Recombinant human XRCC2 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 34.3 kDa (303aa)
• AA Sequence: HHHHH SSGLVPRGSH MGSMCSAFHR AESGTELLAR LEGRSSLKEI EPNLFADEDS PVHGDILEFH GPEGTGKTEM LYHLTARCIL PKSEGGLEVE VLFIDTDYHF DMLRLVTILE HRLSQSSEEI IKYCLGRFFL VYCSSSTHLL LTLYSLESMF CSHPSLCLLI LDSLSAFYWI DRVNGGESVN LQESTLRKCS QCLEKLVNDY RLVLFATTQT IMQKASSSSE EPSHASRRLC DVDIDYRPYL CKAWQQLVKH RMFFSKQDDS QSSNQFSLVS RCLKSNSLKK HFFIIGESGV EFC
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: XRCC2 X-ray repair complementing defective repair in Chinese hamster cells 2 [ Homo sapiens ]
• Official Symbol: XRCC2
• Synonyms: XRCC2; X-ray repair complementing defective repair in Chinese hamster cells 2; DNA repair protein XRCC2; RAD51 like; X-ray repair cross-complementing protein 2; X-ray repair, complementing defective, repair in Chinese hamster; DKFZp781P0919
• Gene ID: 7516
• mRNA Refseq: NM_005431
• Protein Refseq: NP_005422
• MIM: 600375
• UniProt ID: O43543
• Chromosome Location: 7q36
• Pathway: Homologous recombination, organism-specific biosystem; Homologous recombination, conserved biosystem
• Function: ATP binding; DNA binding; DNA-dependent ATPase activity

Citation

Updated protocols for the detection of Sotatercept and Luspatercept in human serum.

Journal: Drug testing and analysis    PubMed ID: 30203930    Data: 2019/4/1

Authors: Christian Reichel, Günter Gmeiner, Mario Thevis

Article Snippet: Sotatercept (Ile21-Pro135 of ACVR2A (115aa; 1-115), TGGG linker (4aa; 116-119), IgG1 fragment (225aa; 120-344) [hinge (120-127), CH2 (128-237), and CH3 (238-344)]) and Luspatercept (Glu25-Thr131 [Leu79>Asp] of ACVR2B (107aa; 1-107), GGG linker (3aa; 108-110), IgG1 fragment (225aa; 111-335) [hinge (111-118), CH2 (119-228), and CH3 (229- 333), CHS (334-335)]) were custom synthesized in CHO-cells by Creative BioMart (Shirley, NY). . Their amino acid sequences are shown in Table 1.Their amino acid sequences are shown in Table 1.