Description : |
Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin. May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes. May recruit PLAA, UBXN6 and VCP to damaged lysosome membranes decorated with K48-linked ubiquitin chains and remove these chains allowing autophagosome formation. |
Source : |
E. coli |
Species : |
Human |
Tag : |
His |
Form : |
Liquid |
Protein Length : |
F2-V348 |
Endotoxin : |
< 0.01 EU per μg of the protein |
Purity : |
80% |
Stability : |
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. |
Storage : |
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Storage Buffer : |
Supplied as sterile. 20mM Tris-HCl (pH 8.0), 150mM NaCl, 10% glycerol |
Shipping : |
It is shipped out with blue ice. |