Description : |
Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Myc/DDK |
Molecular Mass : |
61.3 kDa |
AA Sequence : |
MAAPRPSPAISVSVSAPAFYAPQKK FGPVVAPKPKVNPFRPGDSEPPPAP GAQRAQMGRVGEIPPPPPEDFPLPP PPLAGDGDDAEGALGGAFPPPPPPI EESFPPAPLEEEIFPSPPPPPEEEG GPEAPIPPPPQPREKVSSIDLEIDS LSSLLDDMTKNDPFKARVSSGYVPP PVATPFSSKSSTKPAAGGTAPLPPW KSPSSSQPLPQVPAPAQSQTQFHVQ PQPQPKPQVQLHVQSQTQPVSLANT QPRGPPASSPAPAPKFSPVTPKFTP VASKFSPGAPGGSGSQPNQKLGHPE ALSAGTGSPQPPSFTYAQQREKPRV QEKQHPVPPPAQNQNQVRSPGAPGP LTLKEVEELEQLTQQLMQDMEHPQR QNVAVNELCGRCHQPLARAQPAVRA LGQLFHIACFTCHQCAQQLQGQQFY SLEGAPYCEGCYTDTLEKCNTCGEP ITDRMLRATGKAYHPHCFTCVVCAR PLEGTSFIVDQANRPHCVPDYHKQY APRCSVCSEPIMPEPGRDETVRVVA LDKNFHMKCYKCEDCGKPLSIEADD NGCFPLDGHVLCRKCHTARAQTTRT RPLEQKLISEEDLAANDILDYKDDD DKV |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : |
50 μg/mL as determined by BCA |
Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |