Recombinant HumanMED20, His-tagged

• Cat.No.: MED20-7073H
• Product Overview: Recombinant human MED20 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-212aa
• Description: MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl,50% glycerol, 1mM DTT
• Molecular Mass: 25 kDa (235aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGVTCVS QMPVAEGKSV QQTVELLTRK LEMLGAEKQG TFCVDCETYH TAASTLGSQG QTGKLMYVMHNSEYPLSCFA LFENGPCLIA DTNFDVLMVK LKGFFQSAKA SKIETRGTRY QYCDFLVKVG TVTMGPSARG ISVEVEYGPC VVASDCWSLL LEFLQSFLGS HTPGAPAVFG NRHDAVYGPA DTMVQYMELF NKIRKQQQVP VAGIR
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml

Gene Information

• Gene Name: MED20 mediator complex subunit 20 [ Homo sapiens ]
• Official Symbol: MED20
• Synonyms: MED20; mediator complex subunit 20; Trf (TATA binding protein related factor) proximal homolog (Drosophila) , TRFP; mediator of RNA polymerase II transcription subunit 20; DKFZp586D2223; PRO0213; hTRFP; TRF-proximal protein homolog; Trf (TATA binding protein-related factor)-proximal homolog; TRFP; MGC29869
• Gene ID: 9477
• mRNA Refseq: NM_004275
• Protein Refseq: NP_004266
• MIM: 612915
• UniProt ID: Q9H944
• Chromosome Location: 6p21.1
• Pathway: Developmental Biology, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPARA Activates Gene Expression, organism-specific biosystem
• Function: DNA-directed RNA polymerase activity; RNA polymerase II transcription cofactor activity; protein binding