Recombinant Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) NS2 protein(Met1-Ile121), His-tagged

• Cat.No.: NS2-2424H
• Product Overview: Recombinant influenza A H1N1 Virus (A/Puerto Rico/8/34/Mount Sinai) NS2 protein (P03508.1) (Met 1-Ile 121) was expressed in E. coli, with a polyhistidine tag at the N-terminus.

Specification

• Species: H1N1
• Source: E.coli
• Tag: His
• Protein Length: Met1-Ile121
• Form: Lyophilized from sterile 20mM HEPES, 1mM EDTA, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant influenza A H1N1 Virus (A/Puerto Rico/8/34/Mount Sinai) NS2 protein comprises 121 amino acids and has a predicted molecular mass of 14.4 kDa. It migrates as an approximately 13 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.