| Species : |
Influenza A H3N2 (A/District of Columbia/27/2023) |
| Source : |
Insect Cells |
| Tag : |
His |
| Form : |
Lyophilized from sterile 25 mM sodium citrate, 200 mM NaCl, 0.02% tween80, pH 6.0. |
| Bio-activity : |
1.Immobilized Recombinant Influenza A H3N2(A/District of Columbia/27/2023)HA Protein(His Tag) at 2 μg/mL (100 μL/well) can bind a broadly neutralizing influenza HA stem-specific antibody, the EC50 is 5-15 ng/mL.
2.Immobilized Recombinant Influenza A H3N2(A/District of Columbia/27/2023)HA Protein(His Tag) at 2 μg/mL (100 μL/well) can bind a broadly neutralizing influenza HA stem-specific antibody, the EC50 is 3-10 ng/mL. |
| Molecular Mass : |
The recombinant Influenza A virus ((A/District of Columbia/27/2023) (H3N2))Hemagglutinin / HA comprises 558 amino acids and has a predicted molecular mass of 62.41 kDa. It migrates as an approximately 73.8 kDa band in SDS-PAGE under reducing conditions. |
| Endotoxin : |
< 1.0 EU per μg protein as determined by the LAL method. |
| Purity : |
≥ 95 % as determined by SDS-PAGE. |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
