Cat.No. : |
HA1-348I |
Product Overview : |
Recombinant Influenza A H9N2 (A/Guinea fowl/Hong Kong/WF10/99) HA1 Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Arg 338 (Accession # Q80KD9-1). Predicted N-terminus: Asp 19. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 39.3 kDa. The protein migrates as 47-70 kDa under reducing (R) condition (SDS-PAGE). The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
Source : |
HEK293 |
Species : |
Influenza A H9N2 |
Tag : |
His & Avi |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Molecular Mass : |
39.3 kDa |
Protein length : |
Asp 19 - Arg 338 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>90% as determined by SDS-PAGE. |
Notes : |
Please avoid repeated freeze-thaw cycles. |
Stability : |
No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Figure : |
|