Recombinant Influenza A [Sydney/5/2021 (H1N1)] HA protein, His-tagged

• Cat.No.: HA-571H
• Product Overview: Recombinant Influenza A [Sydney/5/2021 (H1N1)] HA protein(EPI2222932, GISAID)(Asp 18 - Leu 531) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Influenza A [Sydney/5/2021 (H1N1)]
• Source: HEK293
• Tag: His
• Protein Length: 18-531 aa
• Form: Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
• Bio-activity: The His-tagged HA protein from Influenza A strain Sydney/5/2021 (H1N1) immobilized at 1 μg/mL (100 μL/well) demonstrates specific binding to human anti-HA (H1N1) monoclonal antibody 3E1, showing linear dose-dependency within the antibody concentration range of 0.001-0.025 μg/mL.
• Molecular Mass: The protein has a calculated MW of 63.1 kDa.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.