Recombinant Leishmania infantum K39 Antigen, His tagged, Biotinylated

• Cat.No.: K39-028L
• Product Overview: The Leishmania infantum antigen kinesin K39 has been prepared as a recombinant antigen fused to a his-tag in the N-terminus and monobiotinylated in vivo.

Specification

• Species: Leishmania infantum
• Source: E.coli
• Tag: His
• Description: The protein K39 is a repetitive immunodominant epitope in a kinesin-related protein that is highly conserved among viscerotropic Leishmania species. It has been described that a rK39 ELISA is sensitive and specific for serodiagnosis of human and canine VL.
• Tag: N-His
• Conjugation/Label: Biotin
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 35 and 45 kDa, while relative molecular mass calculated from amino acid sequence is 35,241.81 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, CE, DAS, NP, PO
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 8, 1 M NaCl and 0.1% polyoxyethylene (10) tridecyl ether
• Concentration: 1.16 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Scalone, A., de Luna, R., Oliva, G., Baldi, L., Satta, G., Vesco, G., Mignone, W., Turilli, C., Mondesire, R.R., Simpson, D., Donoghue, A.R., Frank, G.R., Gradoni, L. Evaluation of the Leishmania recombinant K39 antigen as a diagnostic marker for canine leishmaniasis and validation of a standardized enzymelinked immunosorbent assay. 2002, Vet. Parasitol. 104:275–285.
  2. Bradford, MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976, 131:499-503.
• Conjugation: Biotin