Recombinant Leptotrichia wadei Cas13a, His-tagged

• Cat.No.: Cas13a-501L
• Product Overview: Recombinant Leptotrichia wadei CRISPR-associated Endoribonuclease Cas13a (Met1-Glu1152), fused with His-tag at the N-terminus, was expressed in E.coli and purified by immobilized metal ion affinity chromatography (IMAC).

Specification

• Species: Leptotrichia wadei
• Source: E.coli
• Tag: His
• Protein Length: 1-1152 a.a.
• Description: Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas13a, previously known as CRISPR-C2c2, is the most recently identified RNA-guided RNA-targeting CRISPR-Cas system and has the unique characteristics of both targeted and collateral single-stranded RNA (ssRNA) cleavage activities, which was first identified in Leptotrichia shahii CRISPR-Cas13a.
• Form: Liquid. Filtered solution in 20mM NaH2PO4 (pH 7.4), 0.5M NaCl, 40mM imidazole, 10% glycerol, 2mM DTT.
• Molecular Mass: 150 kDa
• Purity: >85% by SDS-PAGE under reducing conditions and visualized by Coomassie blue staining.
• Storage: Upon arrival, the protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store at -20 centigrade or -80 centigrade in appropriate aliquots. Avoid repeated freeze-thaw cycles.
• Concentration: Lot specific (see the label on the vial), determined by Bradford protein assay.
• Warning: This product is furnished for LABORATORY RESEARCH USE ONLY.
  Not for diagnostic or therapeutic use.

Gene Information

• Gene Name: Cas13a
• Official Symbol: Cas13a
• Synonyms: CRISPR-associated endoribonuclease
• Protein Refseq: WP_021746003.1
• UniProt ID: U2PWF1