| Cat. No. : |
IL8-5434M |
| Description : |
IL8, also named CXCL8, monocyte-derivedneutrophil chemotactic factor (MDNCF), neutrophil-activating protein 1(NAP1), neutrophil-activating factor (NAF) and granulocyte chemotacticpeptide (GCP), belongs to the Glu-Leu-Arg motif containing (ELR+)CXC chemokine family and has been designated CXCL8. IL8 is a potentneutrophil chemoattractant that recruits neutrophils to sites ofinflammation. IL8 also activates neutrophil functions and promotesangiogenesis. |
| Source : |
E. coli |
| Species : |
Macaca mulatta |
| Form : |
Lyophilized from a0.2µm filtered concentrated solution in PBS, pH 7.4. |
| MolecularWeight : |
Approximately 9.1kDa, a single non-glycosylated polypeptide chain containing 79 amino acids. |
| Purity : |
> 97% by SDS-PAGEand HPLC analyses |
| Endotoxin Level : |
Less than 1EU/µg ofrRh IL8 as determined by LAL method. |
| Biological Activity : |
Determined by itsability to chemoattract human peripheral blood neutrophils using aconcentration range of 25-150 ng/ml. |
| Sequences : |
AVLPRSAKELRCECIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE PWVQRVVEKF VKRAENQNP |
| Reconstitution : |
We recommend thatthis vial be briefly centrifuged prior to opening to bring the contents tothe bottom. Reconstitute in sterile distilled water or aqueous buffercontaining 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutionsshould be apportioned into working aliquots and stored at ≤-20°C. Furtherdilutions should be made in appropriate buffered solutions. |
| Storage : |
This lyophilizedpreparation is stable at 2-8°C, but should be kept at -20°C for long termstorage, preferably desiccated. Upon reconstitution, the preparation isstable for up to one week at 2-8°C. For maximal stability, apportion thereconstituted preparation into working aliquots and store at -20°C to -80°C.Avoid repeated freeze/thaw cycles. |
| OfficialSymbol : |
IL8 |
