Recombinant Mannheimia Haemolytica LKTA Protein (715-953 aa), His-tagged

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Recombinant Mannheimia Haemolytica LKTA Protein (715-953 aa), His-tagged

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Cat.No. : LKTA-1570M
Product Overview : Recombinant Mannheimia Haemolytica (Pasteurella haemolytica) LKTA Protein (715-953 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Partial.
Description : Pasteurella leukotoxins are exotoxins that attack host leukocytes and especially polymorphonuclear cells, by causing cell rupture. The leukotoxin binds to the host LFA-1 integrin and induces a signaling cascade leading to many biological effects, including tyrosine phosphorylation of the CD18 tail, elevation of the intracellular Ca2+ and lysis of the host cell . This leukotoxin is a major contributor to the pathogenesis of lung injury in ovine pneumonic pasteurellosis. It has also weak holytic activity.
Source : Yeast
Species : Mannheimia Haemolytica
Tag : His
Form : Tris-based buffer,50% glycerol
Molecular Mass : 28.0 kDa
Protein length : 715-953 aa
AA Sequence : IGTSHNDIFK GSKFNDAFNG GDGVDTIDGN DGNDRLFGGK GDDIIDGGNG DDFIDGGKGN DLLHGGKGDD IFVHRQGDGN DSITESEGND KLSFSDSNLK DLTFEKVNHH LVITNTKQEK VTIQNWFREA EFAKTIQNYV ATRDDKIEEI IGQNGERITS KQVDELIEKG NGKIAQSELT KVVDNYQLLK YSRDASNSLD KLISSASAFT SSNDSRNVLA SPTSMLDPSL SSIQFARAA
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with reconstitution instruction is sent along with the products.
Synonyms : lktA;
UniProt ID : P0C085
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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