Active Recombinant MERS-CoV (NCoV / Novel coronavirus) S Protein, His-tagged

• Cat.No.: S-598V
• Product Overview: Recombinant extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012)( AFS88936.1) (Met1-Trp1297) was fused with a polyhistidine tag at the C-terminus.

Specification

• Source: Baculovirus-Insect Cells
• Species: MERS-CoV
• Tag: His
• Predicted N Terminal: Tyr 18
• Form: Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its binding ability in a functional ELISA. 1. Immobilized Spike (HCoV-EMC/2012) (ECD, aa 1-1297) at 10 μg/ml (100 μl/well) can bind biotinylated human ACE2-Fc. The EC50 of biotinylated human ACE2-Fc is 0.9-1.4 μg/ml. 2. Immobilized Spike (HCoV
• Molecular Mass: The recombinant extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012) comprises 1289 amino acids and has a predicted molecular mass of 142.2 kDa. It migrates as an approximately 157 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: S S protein [ Middle East respiratory syndrome coronavirus ]
• Official Symbol: S
• Synonyms: spike glycoprotein; S protein
• Gene ID: 14254594