Recombinant MERS-CoV S Protein, His-tagged

• Cat.No.: S-014M
• Product Overview: Recombinant MERS-CoV Spike, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses. MERS-CoV was first identified in the Kingdom of Saudi Arabia in 2012, which is a single and positive stranded RNA virus. Dromedary camels are widely considered as the source of the transmission of MERS-CoV. The rate of human transmission among household contacts of MERS patients has been approximately 5 % based on serological analysis. MERS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. MERS-CoV S mediates viral attachment and fusion to human cells via human cellular receptor DPP4, also known as CD26. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. Recombinant MERS-CoV Spike, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
• Source: Insect cells
• Species: MERS-CoV
• Tag: His
• Form: Liquid
• Molecular Mass: 141.6kDa
• AA Sequence: YVDVGPDSVKSACIEVDIQQTFFDK TWPRPIDVSKADGIIYPQGRTYSNI TITYQGLFPYQGDHGDMYVYSAGHA TGTTPQKLFVANYSQDVKQFANGFV VRIGAAANSTGTVIISPSTSATIRK IYPAFMLGSSVGNFSDGKMGRFFNH TLVLLPDGCGTLLRAFYCILEPRSG NHCPAGNSYTSFATYHTPATDCSDG NYNRNASLNSFKEYFNLRNCTFMYT YNITEDEILEWFGITQTAQGVHLFS SRYVDLYGGNMFQFATLPVYDTIKY YSIIPHSIRSIQSDRKAWAAFYVYK LQPLTFLLDFSVDGYIRRAIDCGFN DLSQLHCSYESFDVESGVYSVSSFE AKPSGSVVEQAEGVECDFSPLLSGT PPQVYNFKRLVFTNCNYNLTKLLSL FSVNDFTCSQISPAAIASNCYSSLI LDYFSYPLSMKSDLSVSSAGPISQF NYKQSFSNPTCLILATVPHNLTTIT KPLKYSYINKCSRLLSDDRTEVPQL VNANQYSPCVSIVPSTVWEDGDYYR KQLSPLEGGGWLVASGSTVAMTEQL QMGFGITVQYGTDTNSVCPKLEFAN DTKIASQLGNCVEYSLYGVSGRGVF QNCTAVGVRQQRFVYDAYQNLVGYY SDDGNYYCLRACVSVPVSVIYDKET KTHATLFGSVACEHISSTMSQYSRS TRSMLKRRDSTYGPLQTPVGCVLGL VNSSLFVEDCKLPLGQSLCALPDTP STLTPRSVRSVPGEMRLASIAFNHP IQVDQLNSSYFKLSIPTNFSFGVTQ EYIQTTIQKVTVDCKQYVCNGFQKC EQLLREYGQFCSKINQALHGANLRQ DDSVRNLFASVKSSQSSPIIPGFGG DFNLTLLEPVSISTGSRSARSAIED LLFDKVTIADPGYMQGYDDCMQQGP ASARDLICAQYVAGYKVLPPLMDVN MEAAYTSSLLGSIAGVGWTAGLSSF AAIPFAQSIFYRLNGVGITQQVLSE NQKLIANKFNQALGAMQTGFTTTNE AFQKVQDAVNNNAQALSKLASELSN TFGAISASIGDIIQRLDVLEQDAQI DRLINGRLTTLNAFVAQQLVRSESA ALSAQLAKDKVNECVKAQSKRSGFC GQGTHIVSFVVNAPNGLYFMHVGYY PSNHIEVVSAYGLCDAANPTNCIAP VNGYFIKTNNTRIVDEWSYTGSSFY APEPITSLNTKYVAPQVTYQNISTN LPPPLLGNSTGIDFQDELDEFFKNV STSIPNFGSLTQINTTLLDLTYEML SLQQVVKALNESYIDLKELGNYTYY NKWP
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 85% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -93 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Bradford assay)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Official Symbol: S
• Synonyms: Middle East respiratory syndrome coronavirus; Human betacoronavirus 2c EMC/2012; MERS-CoV; MERS; MERSCoV SP; Spike glycoprotein; S glycoprotein; S; Spike protein
• UniProt ID: K0BRG7

Gene Information

• Official Symbol: S
• Synonyms: Middle East respiratory syndrome coronavirus; Human betacoronavirus 2c EMC/2012; MERS-CoV; MERS; MERSCoV SP; Spike glycoprotein; S glycoprotein; S; Spike protein
• UniProt ID: K0BRG7