Recombinant MERS-CoV S Protein, His-tagged

• Cat.No.: S-015M
• Product Overview: Recombinant MERS-CoV Spike S1 Subunit, fused to His-tag at C-terminus, was expressed in insect cell, and purified by using conventional chromatography techniques.

Specification

• Species: MERS-CoV
• Source: Insect cells
• Tag: His
• Description: Spike protein S1: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.
• Form: Liquid
• Molecular Mass: 82.0kDa
• AA Sequence: YVDVGPDSVKSACIEVDIQQTFFDKTWPRPIDVSKADGIIYPQGRTYSNITITYQGLFPYQGDHGDMYVYSAGHATGTTPQKLFVANYSQDVKQFANGFVVRIGAAANSTGTVIISPSTSATIRKIYPAFMLGSSVGNFSDGKMGRFFNHTLVLLPDGCGTLLRAFYCILEPRSGNHCPAGNSYTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFMYTYNITEDEILEWFGITQTAQGVHLFSSRYVDLYGGNMFQFATLPVYDTIKYYSIIPHSIRSIQSDRKAWAAFYVYKLQPLTFLLDFSVDGYIRRAIDCGFNDLSQLHCSYESFDVESGVYSVSSFEAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYSYINKCSRLLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQLGNCVEYSLYGVSGRGVFQNCTAVGVRQQRFVYDAYQNLVGYYSDDGNYYCLRACVSVPVSVIYDKETKTHATLFGSVACEHISSTMSQYSRSTRSMLKRRDSTYGPLQTPVGCVLGLVNSSLFVEDCKLPLGQSLCALPDTPSTLTPRSVR
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 85% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -94 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Bradford assay)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Official Symbol: S
• Synonyms: Middle East respiratory syndrome coronavirus; Human betacoronavirus 2c EMC/2012; MERS-CoV; MERS; MERSCoV S1 P; Spike1 glycoprotein; S1 glycoprotein; S1; Spike S1 Subunit protein; S1 Subunit
• UniProt ID: K0BRG7